Phys. Rev. Lett. 87, 148101 (2001) [4 pages]Phase Sensitive X-Ray Diffraction Imaging of Defects in Biological Macromolecular Crystals |
PRL Celebrates 50 Years
This Week's Milestone Letters are from 1974: |
Z. W. Hu1 *, B. Lai2, Y. S. Chu2, Z. Cai2, D. C. Mancini2, B. R. Thomas1,3, and A. A. Chernov1
1Universities Space Research Association, NASA/Marshall Space Flight Center, Huntsville, Alabama 35812
2Advanced Photon Source, Argonne National Laboratory, Argonne, Illinois 60439
3Center for Microgravity Materials Research, University of Alabama in Huntsville, Huntsville, Alabama 35899
Received 7 February 2001; published 12 September 2001
Conventional x-ray diffraction topography is currently used to map defects in the bulk of protein crystals, but the lack of sufficient contrast is frequently a limiting factor. We experimentally demonstrate that this barrier can be circumvented using a method that combines phase sensitive and diffraction imaging principles. Details of defects revealed in tetragonal lysozyme and cubic ferritin crystals are presented and discussed. The approach enabling the detection of the phase changes of diffracted x rays should prove to be useful in the study of defect structures in a broad range of biological macromolecular crystals.
©2001 The American Physical Society
URL: http://link.aps.org/abstract/PRL/v87/e148101
DOI: 10.1103/PhysRevLett.87.148101
PACS: 87.59.-e, 41.60.Ap, 61.72.Ff, 87.14.Ee
* Email address: zhengwei.hu@msfc.nasa.gov
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